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KMID : 0366919980100010265
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Sungkyun Pharmceutical Journal
1998 Volume.10 No. 1 p.265 ~ p.272
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High-performance liquid chromatography with a column-switching system and capillary electrophoresis for the determination of ibuprofen in plasma
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Kang, Seong Ho
Chang, Si-Young/Do, Ki-Chan/Chi, Sang-Cheol/Chung, Doo Soo
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Abstract
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Quantitative aspects of high-performance liquid chromatography with a column-switching system (CSS-HPLC) and capillary electrophoresis (CE) were investigated for the determination of ibuprofen in plasma. For CSS-HPLC, 100μl of plasma was directly injected onto the column system for the three separation steps: ⑴ deproteinization and fractionation of plasma samples with a polymer-coated mixed-function phase column. ⑵ concentration with an intermediate column and ⑶ final separation with a main column. For CE, a mixture of 50μl of plasma and 1㎖ of acetonitrile was centrifuged and the supernatant was introduced onto the capillary (66㎝×50㎛ I.D.; 62㎝ to detector) at 20℃. Run buffer was 250 mM sodium borate buffer (pH 8.5) and applied electric field was 379 V ㎝^-1. Linear dynamic ranges were 0.1-250 ㎍㎖^-1 in CSS-HPLC and 1-1000 ㎍㎖^-1 in CE. Intra-day and inter-day coefficients of variation were less than 5.6% and 6.5% for CSS-HPLC, 6.3% and 6.5% for CE, respectively. The limits of detection (S/N=3) for CSS-HPLC and CE were 25ng㎖^-1 and 300 ng㎖^-1, respectively. CSS-HPLC was superior in simplicity and sensitivity, while CE was better in efficiency, rapidity, and cost.
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